Part:BBa_K3788022

Optimized Timer-lysis device

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1323
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1643
  Illegal BamHI site found at 1316
  Illegal XhoI site found at 1192
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1392
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1146